3HL6
Staphylococcus aureus pathogenicity island 3 ORF9 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 125 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-01-13 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.739, 78.578, 83.541 |
| Unit cell angles | 90.00, 107.41, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.23043 |
| Rwork | 0.228 |
| R-free | 0.27638 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.403 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 17190 | |
| <I/σ(I)> | 22.4 | 2.8 |
| Completeness [%] | 96.7 | 83.8 |
| Redundancy | 3.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 3 M sodium nitrate, 0.1 M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
