3HKF

Murine unglycosylated IgG Fc fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-07-05
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 61 2 2
Unit cell lengths	96.401, 96.401, 90.906
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.500
R-factor	0.246
Rwork	0.242
R-free	0.30510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h3w
RMSD bond length	0.011
RMSD bond angle	1.660
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.056
Number of reflections	9063
Completeness [%]	100.0	98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	2-ethoxyethanol, pH8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K