3HKB
Tubulin: RB3 Stathmin-like domain complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 65 |
| Unit cell lengths | 328.826, 328.826, 53.711 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.650 |
| R-factor | 0.21553 |
| Rwork | 0.213 |
| R-free | 0.25426 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sa0 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.532 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.730 |
| High resolution limit [Å] | 3.650 | 3.650 |
| Number of reflections | 36513 | |
| <I/σ(I)> | 19 | 2.28 |
| Completeness [%] | 96.4 | 96.9 |
| Redundancy | 3.1 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | PEG, PIPES BUFFER, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
