3HJY

Structure of a functional ribonucleoprotein pseudouridine synthase bound to a substrate RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2008-03-16
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	P 62 2 2
Unit cell lengths	238.341, 238.341, 127.369
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.330 - 3.650
R-factor	0.277
Rwork	0.276
R-free	0.30600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ey4
RMSD bond length	0.013
RMSD bond angle	1.505
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall
Low resolution limit [Å]	49.330
High resolution limit [Å]	3.650
Number of reflections	22492

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	303	2.0mM CoCl2, 30mM CaCl2, 2.0mM Spermine, 2.0M LiCl, pH 6.5, vapor diffusion, hanging drop, temperature 303K
1	VAPOR DIFFUSION, HANGING DROP	6.5	303	2.0mM CoCl2, 30mM CaCl2, 2.0mM Spermine, 2.0M LiCl, pH 6.5, vapor diffusion, hanging drop, temperature 303K