3HJP
The crystal structure of Bcp4 from Sulfolobus Solfataricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-04 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.1000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.227, 64.142, 76.692 |
| Unit cell angles | 90.00, 114.76, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.550 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3drn |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Number of reflections | 20646 | |
| <I/σ(I)> | 20 | 2.2 |
| Completeness [%] | 96.1 | 77.4 |
| Redundancy | 3.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.6 | 298 | 0.15 M ammonium acetate, 25% PEG 4000, 0.1 M Sodium acetate , pH 3.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
