3HJI
1.8 Angstrom Crystal Structure of the I74V:I85V Variant of Vivid (VVD).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2008-05-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.400, 80.600, 57.400 |
| Unit cell angles | 90.00, 95.80, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.266 |
| Rwork | 0.244 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pd7 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.600 | 1.830 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.063 | 0.474 |
| Number of reflections | 29885 | |
| <I/σ(I)> | 31 | 2.5 |
| Completeness [%] | 97.8 | 96.6 |
| Redundancy | 4.3 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 3.6 mg/ml protein in buffer containing 5 mM DTT, 100 mM NaCl, 50 mM Hepes pH 8.0 and 10% glycerol combined with equal volume of 28% PEG 4k, 100 mM ammonium acetate, and 100 mM tri-sodium citrate pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
