3HJD
X-ray structure of monomeric variant of HNP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 69.850, 69.850, 46.330 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 1.650 |
| R-factor | 0.17544 |
| Rwork | 0.174 |
| R-free | 0.20313 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dfn |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.011 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0057) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.890 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.094 | 0.476 |
| Number of reflections | 8402 | |
| <I/σ(I)> | 16.4 | 4.2 |
| Completeness [%] | 99.4 | 99.5 |
| Redundancy | 13 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES-Na (pH 7.5), 0.2 M sodium citrate, 30% (v/v) MPD, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
