3HJC
Crystal structure of the carboxy-terminal domain of HSP90 from Leishmania major, LmjF33.0312
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | .97944 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 169.241, 169.241, 83.317 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.216 |
| Rwork | 0.213 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1usu |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.178 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.133 | 0.924 |
| Number of reflections | 25015 | |
| <I/σ(I)> | 27.619 | 4.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 18 | 18.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 2.4 M Ammonium Sulfate 0.1 M Citric Acid pH 5.5 2 mM ATP, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
