3HJ4
Minor Editosome-Associated TUTase 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-11 |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.208, 102.027, 66.074 |
Unit cell angles | 90.00, 111.99, 90.00 |
Refinement procedure
Resolution | 31.410 - 1.560 |
R-factor | 0.164 |
Rwork | 0.162 |
R-free | 0.20300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.368 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 1.620 |
High resolution limit [Å] | 1.560 | 1.560 |
Rmerge | 0.068 | 0.531 |
Number of reflections | 108380 | |
<I/σ(I)> | 25.181 | |
Completeness [%] | 98.2 | 90.3 |
Redundancy | 3.6 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 291 | 0.2M lithium acetate, 25% PEG 3350, pH 8.0, vapor diffusion, temperature 291K |