3HIX
Crystal Structure of the Rhodanese_3 like domain from Anabaena sp Alr3790 protein. Northeast Structural Genomics Consortium Target NsR437i
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-15 |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 62.306, 105.479, 41.615 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.610 - 1.920 |
| R-factor | 0.215 |
| Rwork | 0.214 |
| R-free | 0.23000 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.332 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.086 | 0.529 |
| Number of reflections | 21563 | |
| <I/σ(I)> | 37.6 | 3.9 |
| Completeness [%] | 99.5 | 95.5 |
| Redundancy | 14.2 | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 277 | 20% PEG 4000, 0.1M manganese chloride, 0.1M MES, pH 6.0, Microbatch under paraffin oil, temperature 277K |
