3HIF
The crystal structure of apo wild type CAP at 3.6 A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | .98 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 125.291, 125.291, 224.677 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.060 - 3.590 |
| R-factor | 0.29608 |
| Rwork | 0.295 |
| R-free | 0.31823 |
| Structure solution method | AVERAGING |
| Starting model (for MR) | cAMP Binding Domain of CAP |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.893 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | DM |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.060 | 3.730 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.456 | |
| Number of reflections | 22948 | |
| <I/σ(I)> | 23.05 | 2.621 |
| Completeness [%] | 98.9 | |
| Redundancy | 6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | Protein was crystallized after it was concentrated to ~25 mg/ml and seeded with apo D138L mutant CAP., pH 7.5, VAPOR DIFFUSION, temperature 298K |
