3HI8
Crystal structure of proliferating cell nuclear antigen (PCNA) from Haloferax volcanii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.8726 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 174.443, 123.236, 123.256 |
| Unit cell angles | 90.00, 135.00, 90.00 |
Refinement procedure
| Resolution | 32.949 - 3.202 |
| Rwork | 0.237 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rwz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.495 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.949 | 3.360 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 26380 | |
| <I/σ(I)> | 4.4 | 1.02 |
| Completeness [%] | 99.3 | 86.3 |
| Redundancy | 2.2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 4M Sodium malonate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
