3HHZ
Complex of the vesicular stomatitis virus nucleocapsid and the nucleocapsid-binding domain of the phosphoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-25 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 166.568, 235.248, 96.138 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.500 |
| R-factor | 0.25788 |
| Rwork | 0.254 |
| R-free | 0.32333 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gic |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.194 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | COMO |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.630 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.116 | 0.434 |
| Number of reflections | 48185 | |
| <I/σ(I)> | 21.48 | 4.31 |
| Completeness [%] | 98.3 | 88.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 295 | 7% PEG 4000, 0.25 M NaCl, and 0.1M citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
