3HHS
Crystal Structure of Manduca sexta prophenoloxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-02 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 148.832, 153.664, 75.763 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.450 - 1.970 |
| R-factor | 0.156 |
| Rwork | 0.154 |
| R-free | 0.19400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.516 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.3) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
| High resolution limit [Å] | 1.970 | 5.430 | 1.970 |
| Rmerge | 0.105 | 0.038 | 0.928 |
| Number of reflections | 121340 | ||
| <I/σ(I)> | 28.646 | ||
| Completeness [%] | 100.0 | 99.2 | 100 |
| Redundancy | 8.3 | 7.9 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sitting drop | 7.5 | 298 | PEG10000, pH 7.5, sitting drop, temperature 298K |
