3HHJ
Crystal structure of mutator mutT from Bartonella henselae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.500, 93.490, 43.880 |
| Unit cell angles | 90.00, 109.24, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.203 |
| Rwork | 0.202 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fk9 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.391 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.073 | 0.488 |
| Number of reflections | 21194 | 1406 |
| <I/σ(I)> | 12.71 | 2.06 |
| Completeness [%] | 98.4 | 88.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | Proplex sparse matrix screen condition B5, 0.1 M Hepes pH 7.5, 10% PEG 4000, 0.1M MgCl2, 20% Glycerol as cryo, 26.9 mg/mL Protein, crystal tracking ID 202117b5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
