3HHF

Structure of CrgA regulatory domain, a LysR-type transcriptional regulator from Neisseria meningitidis.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9780, 0.9065, 0.9785, 0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	51.600, 65.600, 63.500
Unit cell angles	90.00, 105.00, 90.00

Refinement procedure

Resolution	30.000 - 2.300
R-factor	0.20417
Rwork	0.201
R-free	0.25819
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.000
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.370
High resolution limit [Å]	2.300	2.290
Rmerge	0.073	0.299
Number of reflections	15951
<I/σ(I)>	18	2.1
Completeness [%]	85.8	34.8
Redundancy	4.1	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	200 mM ammonium acetate, 25% PEG 3350, 0.1M bis-Tris pH5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	200 mM ammonium acetate, 25% PEG 3350, 0.1M bis-Tris pH5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	200 mM ammonium acetate, 25% PEG 3350, 0.1M bis-Tris pH5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K