3HH1
The Structure of a Tetrapyrrole methylase family protein domain from Chlorobium tepidum TLS
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97945, 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.986, 47.569, 64.497 |
| Unit cell angles | 90.00, 91.00, 90.00 |
Refinement procedure
| Resolution | 37.960 - 1.850 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.22700 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.487 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.090 | 0.853 |
| Number of reflections | 35606 | |
| <I/σ(I)> | 20.761 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.6 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 297 | 0.2M Ammonium Sulfate, 20% PEG 3350, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
