3HGQ
Structural and functional studies of the yeast class II Hda1 HDAC complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2008-10-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97815, 0.97876, 0.97469 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.080, 132.580, 90.170 |
| Unit cell angles | 90.00, 92.38, 90.00 |
Refinement procedure
| Resolution | 19.872 - 3.000 |
| R-factor | 0.2267 |
| Rwork | 0.224 |
| R-free | 0.27980 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.904 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.120 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 28294 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |
