3HGF
Expression, purification, spectroscopical and crystallographical studies of segments of the nucleotide binding domain of the reticulocyte binding protein Py235 of Plasmodium yoelii
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL12B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL12B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97898 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 70.223, 70.223, 193.218 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.000 - 4.000 |
| R-factor | 0.34864 |
| Rwork | 0.339 |
| R-free | 0.37324 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.839 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.040 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rmerge | 0.102 | 0.393 |
| Number of reflections | 2825 | |
| <I/σ(I)> | 13.712 | 3.69 |
| Completeness [%] | 95.6 | 97.1 |
| Redundancy | 15.4 | 12.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | 35% (v/v) 2-methyl-2,4-pentanediol, 0.1M acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
