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3HGB

Crystal structure of glycine cleavage system protein H from Mycobacterium tuberculosis

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU FR-E+ SUPERBRIGHT
Temperature [K]100
Detector technologyCCD
Collection date2009-05-07
DetectorRIGAKU SATURN 944+
Wavelength(s)1.5418
Spacegroup nameC 1 2 1
Unit cell lengths86.860, 51.470, 32.530
Unit cell angles90.00, 94.64, 90.00
Refinement procedure
Resolution19.240 - 1.750
R-factor0.183
Rwork0.181
R-free0.22000
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1onl
RMSD bond length0.008
RMSD bond angle1.185
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwareMOLREP
Refinement softwareREFMAC
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]19.2401.800
High resolution limit [Å]1.7501.750
Rmerge0.0270.088
Number of reflections140911067
<I/σ(I)>23.756.2
Completeness [%]96.998.1
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP7.5289JCSG+ screen condition B11, 1.6M Na citrate, 27.2 mg/mL protein, tracking ID 202071b11, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K

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