3HGB
Crystal structure of glycine cleavage system protein H from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-07 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.860, 51.470, 32.530 |
| Unit cell angles | 90.00, 94.64, 90.00 |
Refinement procedure
| Resolution | 19.240 - 1.750 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1onl |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.185 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.240 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.027 | 0.088 |
| Number of reflections | 14091 | 1067 |
| <I/σ(I)> | 23.75 | 6.2 |
| Completeness [%] | 96.9 | 98.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | JCSG+ screen condition B11, 1.6M Na citrate, 27.2 mg/mL protein, tracking ID 202071b11, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
