3HG9
CRYSTAL STRUCTURE OF putative pilM protein from Pseudomonas aeruginosa 2192
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-30 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 62 |
| Unit cell lengths | 64.516, 64.516, 143.134 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.236 |
| Rwork | 0.235 |
| R-free | 0.26700 |
| Structure solution method | SAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.450 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 5.170 | 2.400 |
| Rmerge | 0.101 | 0.057 | 0.551 |
| Number of reflections | 24848 | ||
| <I/σ(I)> | 30.543 | ||
| Completeness [%] | 95.1 | 99.4 | 66.5 |
| Redundancy | 10 | 11.2 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.1 M Bis-Tris, 2.0 M Ammonium sulfate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
