3HG0
Crystal structure of a DARPin in complex with ORF49 from Lactococcal phage TP901-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-18 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.650, 80.440, 182.870 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.890 - 2.100 |
R-factor | 0.209 |
Rwork | 0.208 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2JAB AND 2FOC |
RMSD bond length | 0.014 |
RMSD bond angle | 1.676 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | AMoRE |
Refinement software | PHENIX ((phenix.refine: 1.4_56)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.890 | 2.300 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.065 | 0.502 |
Number of reflections | 39881 | |
<I/σ(I)> | 12.43 | 2.48 |
Completeness [%] | 97.0 | 91.8 |
Redundancy | 3.58 | 3.67 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 293 | 40 mM Tris, 20 mM Bis-Tris-Propane, 25% PEG 2000 MME, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |