3HFZ
Crystal structure of Thermus thermophilus Phenylalanyl-tRNA synthetase complexed with m-tyrosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2008-02-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 173.181, 173.181, 138.852 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.040 - 2.900 |
| R-factor | 0.27 |
| Rwork | 0.269 |
| R-free | 0.28200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1pys |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.899 |
| Number of reflections | 3671 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 292 | 30% (NH4)2SO4, 0.02M IMIDAZOLE-HCL, 0.001M magnesium chloride, m-tyrosine, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
