3HFV

Crystal structure of human mitochondrial phenylalanyl-tRNA synthetase complexed with m-tyrosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	200
Detector technology	CCD
Collection date	2008-02-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.93
Spacegroup name	P 21 21 21
Unit cell lengths	54.640, 90.143, 97.133
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.700 - 2.600
R-factor	0.1944
Rwork	0.192
R-free	0.23939
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cmq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	REFMAC (5.4.0067)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.700	2.665
High resolution limit [Å]	2.600	2.597
Number of reflections	14667
Completeness [%]		93.19

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	292	1.8M Ammonium acetate, 0.1M Tris-Propane, 0.1M Magnesium chloride, 0,05M DTT, VAPOR DIFFUSION, HANGING DROP, temperature 292K