3HFN
Crystal Structure of an Hfq protein from Anabaena sp.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-03-03 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 3 |
| Unit cell lengths | 60.580, 60.580, 32.470 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.290 - 2.310 |
| R-factor | 0.273 |
| Rwork | 0.272 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u1t |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.547 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.1.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.300 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.065 | 0.179 |
| Number of reflections | 5806 | 648 |
| <I/σ(I)> | 14.55 | 6.1 |
| Completeness [%] | 97.9 | 90.6 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 277 | 2 M ammonium sulphate, 0.1 M citric acid, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
