3HFD
Nucleosome Assembly Protein 1 from Plasmodium knowlesi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-05-22 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.895, 38.259, 80.477 |
| Unit cell angles | 90.00, 100.41, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.23 |
| Rwork | 0.225 |
| R-free | 0.32800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.182 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 79.060 | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 6.020 | 2.800 |
| Rmerge | 0.068 | 0.031 | 0.214 |
| Number of reflections | 5581 | ||
| <I/σ(I)> | 16.551 | ||
| Completeness [%] | 95.6 | 99.8 | 77.2 |
| Redundancy | 3.4 | 3.4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | 0.1 M NaCl, 0.1 M Tris, 32% PEG 3350, pH 8.0, vapor diffusion, temperature 293K |
