3HEP
Ferric Horse Heart Myoglobin; H64V Mutant, Nitrite Modified
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-03-06 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 30.389, 119.390, 57.155 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.200 - 1.950 |
| R-factor | 0.20045 |
| Rwork | 0.198 |
| R-free | 0.25007 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hc9 |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.095 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.150 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.125 | 0.503 |
| Number of reflections | 15767 | |
| <I/σ(I)> | 7.1 | 2.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.99 | 7.07 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 295 | ammonium phosphate, EDTA, crystal soaking with sodium nitrite, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
