3HEO
Ferric Horse Heart Myoglobin; H64V/V67R mutant, Nitrite Modified
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-03-05 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 29.841, 118.053, 57.363 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.710 - 2.000 |
| R-factor | 0.20457 |
| Rwork | 0.202 |
| R-free | 0.25791 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 3HEN |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.129 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.830 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.095 | 0.571 |
| Number of reflections | 13595 | |
| <I/σ(I)> | 9.9 | 3.3 |
| Completeness [%] | 93.9 | 91 |
| Redundancy | 7.23 | 7.17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 295 | ammonium phosphate, EDTA, crystal soaking with sodium nitrite, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
