3HEE
Structural study of Clostridium thermocellum Ribose-5-Phosphate Isomerase B and ribose-5-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-18 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 69.381, 69.381, 153.566 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.840 - 2.000 |
| R-factor | 0.17668 |
| Rwork | 0.174 |
| R-free | 0.22051 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vvr |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.192 |
| Phasing software | swiss-model |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.095 | 0.095 |
| Number of reflections | 29281 | |
| <I/σ(I)> | 31.6 | 31.6 |
| Completeness [%] | 98.3 | 98.3 |
| Redundancy | 16.3 | 16.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 283 | 0.05M Tris, pH7.0, 10% PEG8000, 0.15M Magnesium chloride, 0.2M Potassium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
