3HEB
Crystal Structure of Response regulator receiver domain from Rhodospirillum rubrum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.121, 75.071, 48.571 |
| Unit cell angles | 90.00, 96.41, 90.00 |
Refinement procedure
| Resolution | 35.210 - 2.400 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.25500 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.470 |
| High resolution limit [Å] | 2.380 | |
| Rmerge | 0.120 | 0.320 |
| Number of reflections | 11105 | |
| <I/σ(I)> | 7.6 | 8.7 |
| Completeness [%] | 98.5 | 84.6 |
| Redundancy | 6.7 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.2 M NaCl, 0.1 M Bis Tris pH 6.5, 25% Peg 3350, 3.0 M NDSB-195, 20 % Glycerol , VAPOR DIFFUSION, SITTING DROP, temperature 298 K |
