3HDY
Crystal Structure of UDP-galactopyranose mutase (reduced form) in complex with substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Detector technology | CCD |
| Collection date | 2009-02-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 132.820, 174.628, 218.192 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.940 - 2.400 |
| R-factor | 0.2377 |
| Rwork | 0.235 |
| R-free | 0.28010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v0j |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.749 |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.940 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.187 | 0.635 |
| Number of reflections | 189435 | |
| <I/σ(I)> | 5.1 | 1.7 |
| Completeness [%] | 95.8 | 92.1 |
| Redundancy | 4.7 | 4.34 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 295 | 0.1 M HEPES pH 6.5, 0.2 M LiCl and 28 % PEG 6000, Microbatch, temperature 295K |
