3HDQ

Crystal structure of UDP-galactopyranose mutase (oxidized form) in complex with substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-09-06
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9797
Spacegroup name	P 21 21 21
Unit cell lengths	134.020, 176.650, 220.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.880 - 2.360
R-factor	0.1793
Rwork	0.177
R-free	0.22630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1v0j
RMSD bond length	0.007
RMSD bond angle	1.152
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall
Low resolution limit [Å]	19.880
High resolution limit [Å]	2.360
Rmerge	0.116
<I/σ(I)>	13.07
Completeness [%]	95.8
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		0.1 M HEPES pH 6.5, 0.2 M LiCl, 28% PEG 6000, Microbatch