3HDO
Crystal Structure of a Histidinol-phosphate aminotransferase from Geobacter metallireducens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.370, 90.075, 77.522 |
| Unit cell angles | 90.00, 92.39, 90.00 |
Refinement procedure
| Resolution | 38.930 - 1.610 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.20200 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.670 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.065 | 0.211 |
| Number of reflections | 81522 | |
| <I/σ(I)> | 15.6 | 2 |
| Completeness [%] | 99.1 | 100 |
| Redundancy | 7.4 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M Potassium Thiocyanate, 30% w/v Polyethylene Glycol monomethyl ether 2000, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
