3HDC
The crystal structure of thioredoxin protein from Geobacter metallireducens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 63.678, 63.678, 94.587 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.900 - 1.771 |
| R-factor | 0.1798 |
| Rwork | 0.179 |
| R-free | 0.19660 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.060 | 0.378 |
| Number of reflections | 21827 | |
| <I/σ(I)> | 15.6 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.5 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 0.1 M Citric acid pH 3.5, 3.0 M Sodium chloride, VAPOR DIFFUSION, SITTING DROP |
