3HD6
Crystal Structure of the Human Rhesus Glycoprotein RhCG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2008-12-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.11587 |
| Spacegroup name | P 63 |
| Unit cell lengths | 99.830, 99.830, 101.076 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 86.390 - 2.100 |
| R-factor | 0.16973 |
| Rwork | 0.168 |
| R-free | 0.19505 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b9w |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.018 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.390 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.077 | 0.589 |
| Number of reflections | 33449 | |
| <I/σ(I)> | 39.7 | 3.4 |
| Completeness [%] | 99.7 | 95.8 |
| Redundancy | 10.6 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.1M Tris pH 8.5 (at 293K), 40% PEG 200 + 20mM Tris pH 7.4 (at 293K), 100mM NaCl, 40mM BOG, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
