3HCV
Crystal structure of HLA-B*2709 complexed with the double citrullinated vasoactive intestinal peptide type 1 receptor (VIPR) peptide (residues 400-408)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.982 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.176, 82.428, 67.017 |
| Unit cell angles | 90.00, 110.15, 90.00 |
Refinement procedure
| Resolution | 48.040 - 1.950 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ogt |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.130 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 | 0.432 |
| Number of reflections | 39804 | |
| <I/σ(I)> | 2.49 | |
| Completeness [%] | 82.8 | 91.9 |
| Redundancy | 3.75 | 3.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.1M TRIS HCL,25% PEG 8000, , pH 8.000000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
