3HCU
Crystal structure of TRAF6 in complex with Ubc13 in the C2 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97912 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.493, 41.387, 123.745 |
| Unit cell angles | 90.00, 116.20, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| R-factor | 0.2556 |
| Rwork | 0.256 |
| R-free | 0.33290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hct |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
| Rmerge | 0.046 | 0.032 | 0.394 |
| Number of reflections | 18484 | ||
| <I/σ(I)> | 55.287 | ||
| Completeness [%] | 98.8 | 98.6 | 94.1 |
| Redundancy | 6.8 | 6.8 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 16-20% PEG 4000, 0.2M Magnesium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
