3HCG
Structure of the C-terminal domain (MsrB) of Neisseria meningitidis PilB (reduced form)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-01-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9791, 0.9796, 0.9765 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.663, 117.232, 137.686 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.590 - 1.820 |
| R-factor | 0.188 |
| Rwork | 0.185 |
| R-free | 0.23500 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.407 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Number of reflections | 62578 | |
| <I/σ(I)> | 12.7 | 3.1 |
| Completeness [%] | 97.2 | 91.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0,5M KH2PO4, 0.1M TRIS HCl pH 8 , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
