3HC9
Ferric Horse Heart Myoglobin; H64V mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-01-02 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 30.639, 119.236, 56.677 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.800 - 2.000 |
| R-factor | 0.18847 |
| Rwork | 0.186 |
| R-free | 0.23727 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Model was generated by Balbes |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.097 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.800 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.098 | 0.490 |
| Number of reflections | 15752 | |
| <I/σ(I)> | 9.8 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.42 | 7.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 295 | ammonium phosphate, EDTA, horse heart myoglobin H64V mutant, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
