3HC4
BHA10 IgG1 Fab quadruple mutant variant - antibody directed at human LTBR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 73.605, 118.233, 126.452 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.620 |
| R-factor | 0.16202 |
| Rwork | 0.161 |
| R-free | 0.18048 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hc0 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.311 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.089 | 0.479 |
| Number of reflections | 66764 | |
| Completeness [%] | 99.5 | 93.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 20% PEG 4000, 200 mM calcium acetate 10 mM zinc chloride and 100 mM Tris at pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
