3HBX

Crystal structure of GAD1 from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-26
Detector	MAR CCD 165 mm
Wavelength(s)	0.90002
Spacegroup name	P 32
Unit cell lengths	118.098, 118.098, 200.576
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.091 - 2.672
R-factor	0.2084
Rwork	0.208
R-free	0.22130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1PMM (E. coli GadB)
RMSD bond length	0.002
RMSD bond angle	0.606
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.4_29)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.740
High resolution limit [Å]	2.650	2.650
Number of reflections	85232
<I/σ(I)>	8	1.7
Completeness [%]	96.4	95.1
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2 uL protein solution (1.9 mg/ml Gad1, 50 mM Hepes pH 7.2, 150 mM NaCl and 10 uM PLP) + 1 uL reservoir solution (100 mM Na acetate pH 5.5, 720 mM Na formate, 9 % PEG 8000 and 9 % PEG 1000), VAPOR DIFFUSION, SITTING DROP, temperature 293K