3HBT
The structure of native G-actin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 95.731, 95.731, 96.948 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.640 - 2.700 |
| R-factor | 0.20484 |
| Rwork | 0.202 |
| R-free | 0.25676 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gwj |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.340 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.089 | 0.400 |
| Number of reflections | 13778 | |
| <I/σ(I)> | 10.8 | 1.6 |
| Completeness [%] | 97.1 | 93.1 |
| Redundancy | 2.6 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 2M ammonium sulfate, 100mM Tris-HCl, pH8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
