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3HBT

The structure of native G-actin

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSRRC BEAMLINE BL13B1
Synchrotron siteNSRRC
BeamlineBL13B1
Temperature [K]100
Detector technologyCCD
DetectorADSC QUANTUM 315
Wavelength(s)1.0
Spacegroup nameP 32 2 1
Unit cell lengths95.731, 95.731, 96.948
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution27.640 - 2.700
R-factor0.20484
Rwork0.202
R-free0.25676
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)2gwj
RMSD bond length0.011
RMSD bond angle1.340
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwareMOLREP
Refinement softwareREFMAC (5.2.0019)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.800
High resolution limit [Å]2.7002.700
Rmerge0.0890.400
Number of reflections13778
<I/σ(I)>10.81.6
Completeness [%]97.193.1
Redundancy2.61.8
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP82952M ammonium sulfate, 100mM Tris-HCl, pH8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K

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PDB entries from 2024-05-15

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