3HBJ
Structure of UGT78G1 complexed with UDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2006-07-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9793 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 85.120, 77.770, 70.025 |
Unit cell angles | 90.00, 93.36, 90.00 |
Refinement procedure
Resolution | 42.490 - 2.100 |
Rwork | 0.209 |
R-free | 0.28100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.800 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.053 | 0.334 |
Number of reflections | 25664 | |
<I/σ(I)> | 19.8 | 2.7 |
Completeness [%] | 95.9 | 87.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 25% PEG 3350, 0.1M Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |