3HAR
Crystal structure of bacteriorhodopsin mutant I148V crystallized from bicelles
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 45.078, 102.418, 128.380 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.230 - 1.700 |
R-factor | 0.183 |
Rwork | 0.181 |
R-free | 0.20600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.452 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 95.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.120 | 0.400 |
Number of reflections | 31173 | |
<I/σ(I)> | 16.38 | |
Completeness [%] | 93.9 | 60.6 |
Redundancy | 9 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop, bicelle method | 3.8 | 310 | 1.92 M sodium phosphate, 180 mM 1,6-hexanediol, 3.5 % triethylene glycol, PFPC used as cryoprotectant, pH 3.8, hanging drop, bicelle method, temperature 310K |