3HAN
Crystal structure of bacteriorhodopsin mutant V49A crystallized from bicelles
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 44.948, 102.878, 128.422 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.200 - 2.750 |
| R-factor | 0.227 |
| Rwork | 0.222 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.037 |
| RMSD bond angle | 2.860 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.105 | 0.382 |
| Number of reflections | 7285 | |
| <I/σ(I)> | 10.238 | |
| Completeness [%] | 89.9 | 52.8 |
| Redundancy | 5.8 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop, bicelle method | 310 | 1 M sodium phosphate (pH 3.9), 1 M sodium phosphate (pH 4.5), 180 mM 1,6-hexanediol, 3.5% triethyleneglycol, PFPC used as cryoprotectant, hanging drop, bicelle method, temperature 310K |
