3HAL
Crystal structure of Rabbit acidic fibroblast growth factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2009-04-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.182, 44.737, 67.051 |
| Unit cell angles | 90.00, 110.55, 90.00 |
Refinement procedure
| Resolution | 31.911 - 1.800 |
| R-factor | 0.185 |
| Rwork | 0.182 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jqz |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.806 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.430 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.069 | 0.384 |
| Number of reflections | 24147 | |
| <I/σ(I)> | 36.07 | 3.93 |
| Completeness [%] | 94.2 | 99.8 |
| Redundancy | 4.3 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 30% PEG4000, 0.1M Na HEPES (pH7.5), 0.2M NaOAc, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
