3HAJ

Crystal structure of human PACSIN2 F-BAR domain (p212121 lattice)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	110
Detector technology	CCD
Collection date	2008-07-09
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	31.295, 88.395, 357.722
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.715 - 2.780
R-factor	0.2265
Rwork	0.224
R-free	0.27350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hai
RMSD bond length	0.003
RMSD bond angle	0.672
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.720	2.950
High resolution limit [Å]	2.780	2.780
<I/σ(I)>	14.2	2.8
Completeness [%]		30.2
Redundancy	4.2	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	20% PEG-MME 5000, 0.1 M Bis-Tris pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K