3HAI
Crystal structure of human PACSIN1 F-BAR domain (P21 lattice)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2007-12-07 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 163.620, 32.160, 181.160 |
| Unit cell angles | 90.00, 111.23, 90.00 |
Refinement procedure
| Resolution | 48.520 - 2.881 |
| R-factor | 0.2843 |
| Rwork | 0.282 |
| R-free | 0.31850 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.166 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.520 | 3.060 |
| High resolution limit [Å] | 2.880 | 2.880 |
| <I/σ(I)> | 9.9 | 2.1 |
| Completeness [%] | 41.2 | |
| Redundancy | 2.6 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M Hepes pH 7.0, 18% PEG3350, 1% Tryptone, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
