3H9W
Crystal Structure of the N-terminal domain of Diguanylate cyclase with PAS/PAC sensor (Maqu_2914) from Marinobacter aquaeolei, Northeast Structural Genomics Consortium Target MqR66C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97904 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 55.531, 55.531, 136.238 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.640 - 1.900 |
| R-factor | 0.97 |
| Rwork | 0.196 |
| R-free | 0.22100 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.073 | 0.489 |
| Number of reflections | 18593 | |
| <I/σ(I)> | 33.77 | 4.41 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.2 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch under oil | 6.5 | 291 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5) . Reservoir solution: 0.1M bis-tris (pH 6.5), 25% PEG3350, and 0.2M NaCl. , Microbatch under oil, temperature 291K |
