3H9U
S-adenosyl homocysteine hydrolase (SAHH) from Trypanosoma brucei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97930 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.350, 136.050, 191.870 |
| Unit cell angles | 90.00, 99.32, 90.00 |
Refinement procedure
| Resolution | 45.550 - 1.900 |
| R-factor | 0.19815 |
| Rwork | 0.196 |
| R-free | 0.23060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gu1 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.508 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.550 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 181285 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | VAPOR DIFFUSION, SITTING DROP |
